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mouse monoclonal antibody targeting nf κb p65  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse monoclonal antibody targeting nf κb p65
    Mouse Monoclonal Antibody Targeting Nf κb P65, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 14412 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/nf%CE%BAb+p65/NF%CE%BAB+p65+Antibody/pmc12914681-155-24-37
    Average 96 stars, based on 14412 article reviews
    mouse monoclonal antibody targeting nf κb p65 - by Bioz Stars, 2026-09
    96/100 stars

    Images

    Related Articles

    Control:

    Article Title: Exploring the immunomodulatory effects of environmental contaminants on autoimmune patients: An in vitro approach
    Article Snippet: Following washes, membranes were incubated with HRP-conjugated secondary antibodies, and signals were detected using PierceTM ECL substrate (Thermo Scientific, Waltham, MA, USA) on a ChemiDocTM MP imaging system (Bio-Rad, Hercules, CA, USA). .. Membranes were stripped and re-probed for total AKT, NFκB p65, p38 MAPK, STAT1, STAT3, and β-actin (Cell Signaling Technology, Danvers, MA, USA; Santa Cruz Biotechnology, Dallas, TX, USA) as a loading control. ..

    Blocking Assay:

    Article Title: Cellular Anti-Apoptotic Effects of Dapagliflozin in Methotrexate-Induced Liver Toxicity: Bax/Bcl-2/Cyt-C/Cas-9/Cas-3 Signaling Pathway
    Article Snippet: .. After 5 μ thick sections were taken from the paraffin blocks, they were deparaffinized and passed through alcohol series and boiled in citrate buffer solution for 20 min. https://doi.org/10.3390/ijms27052110 After 5 min treatment with H2O2 (TA-060-HP, Lab Vision Corporation, Fremont, CA, USA) and Ultra V Block (TA-125-UB, Thermo Scientific, Cheshire, UK) solution, they were incubated with Caspase-3 (sc-65497, Santa Cruz, CA, USA, 1:200), VEGF (sc-7269, Santa Cruz, CA, USA, 1:200), TNF alpha (sc-52746, Santa Cruz, CA, USA, 1:200), NFκB p65 (sc-8008, Santa Cruz, CA, USA, 1:200), and IL-1β (sc-52012, Santa Cruz, CA, USA, 1:200) for 60 min. Then they were incubated with secondary antibody (Biotinylated Goat AntiPolyvalent TP-125-BN, Thermo Scientific, Cheshire, UK) and Strepavidin HRP (Horse radish peroxidase) (TS-125-HR, Thermo Scientific, Cheshire, UK) for 30 min at room temperature. .. After DAB (3,3′-diaminobenzidine) (TA-125 HD, Thermo Scientific, Cheshire, UK) solution, tissues were counter-stained with Mayer’s Hematoxylin and covered with Entellan.

    Article Title: Cellular Anti-Apoptotic Effects of Dapagliflozin in Methotrexate-Induced Liver Toxicity: Bax/Bcl-2/Cyt-C/Cas-9/Cas-3 Signaling Pathway
    Article Snippet: .. After 5 μ thick sections were taken from the paraffin blocks, they were deparaffinized and passed through alcohol series and boiled in citrate buffer solution for 20 min. After 5 min treatment with H 2 O 2 (TA-060-HP, Lab Vision Corporation, Fremont, CA, USA) and Ultra V Block (TA-125-UB, Thermo Scientific, Cheshire, UK) solution, they were incubated with Caspase-3 (sc-65497, Santa Cruz, CA, USA, 1:200), VEGF (sc-7269, Santa Cruz, CA, USA, 1:200), TNF alpha (sc-52746, Santa Cruz, CA, USA, 1:200), NFκB p65 (sc-8008, Santa Cruz, CA, USA, 1:200), and IL-1β (sc-52012, Santa Cruz, CA, USA, 1:200) for 60 min. Then they were incubated with secondary antibody (Biotinylated Goat Anti-Polyvalent TP-125-BN, Thermo Scientific, Cheshire, UK) and Strepavidin HRP (Horse radish peroxidase) (TS-125-HR, Thermo Scientific, Cheshire, UK) for 30 min at room temperature. .. After DAB (3,3′-diaminobenzidine) (TA-125 HD, Thermo Scientific, Cheshire, UK) solution, tissues were counter-stained with Mayer’s Hematoxylin and covered with Entellan.

    Incubation:

    Article Title: Cellular Anti-Apoptotic Effects of Dapagliflozin in Methotrexate-Induced Liver Toxicity: Bax/Bcl-2/Cyt-C/Cas-9/Cas-3 Signaling Pathway
    Article Snippet: .. After 5 μ thick sections were taken from the paraffin blocks, they were deparaffinized and passed through alcohol series and boiled in citrate buffer solution for 20 min. https://doi.org/10.3390/ijms27052110 After 5 min treatment with H2O2 (TA-060-HP, Lab Vision Corporation, Fremont, CA, USA) and Ultra V Block (TA-125-UB, Thermo Scientific, Cheshire, UK) solution, they were incubated with Caspase-3 (sc-65497, Santa Cruz, CA, USA, 1:200), VEGF (sc-7269, Santa Cruz, CA, USA, 1:200), TNF alpha (sc-52746, Santa Cruz, CA, USA, 1:200), NFκB p65 (sc-8008, Santa Cruz, CA, USA, 1:200), and IL-1β (sc-52012, Santa Cruz, CA, USA, 1:200) for 60 min. Then they were incubated with secondary antibody (Biotinylated Goat AntiPolyvalent TP-125-BN, Thermo Scientific, Cheshire, UK) and Strepavidin HRP (Horse radish peroxidase) (TS-125-HR, Thermo Scientific, Cheshire, UK) for 30 min at room temperature. .. After DAB (3,3′-diaminobenzidine) (TA-125 HD, Thermo Scientific, Cheshire, UK) solution, tissues were counter-stained with Mayer’s Hematoxylin and covered with Entellan.

    Article Title: Viniferin-Rich Phytocomplex from Vitis vinifera L. Plant Cell Culture Mitigates Neuroinflammation in BV2 Microglia Cells
    Article Snippet: Protein samples (20 μg) were separated on 10% SDS-PAGE [ ] and then blotted onto Midi Nitrocellulose membranes using a Trans-Blot Turbo Transfer Starter System (Bio-Rad Laboratories, Milan, Italy). .. Blots were incubated overnight at 4 °C with primary antibodies against pERK1/2 (1:1000, Cell Signaling Technology, Danvers, MA, USA), SIRT1 (1:1000, Santa Cruz Biotechnology, Dallas, TX, USA), NFκB p65 (1:1000, Santa Cruz Biotechnology), and p-NFκB p65 (1:500, Santa Cruz Biotechnology). .. After being washed with PBS containing 0.1% Tween, the nitrocellulose membranes were incubated with goat anti-rabbit or anti-mouse horseradish peroxidase-conjugated secondary antibodies (1:3000, Jackson ImmunoResearch Labs, West Grove, PA, USA) for 2 h at room temperature (RT; 20–22 °C).

    Article Title: Cellular Anti-Apoptotic Effects of Dapagliflozin in Methotrexate-Induced Liver Toxicity: Bax/Bcl-2/Cyt-C/Cas-9/Cas-3 Signaling Pathway
    Article Snippet: .. After 5 μ thick sections were taken from the paraffin blocks, they were deparaffinized and passed through alcohol series and boiled in citrate buffer solution for 20 min. After 5 min treatment with H 2 O 2 (TA-060-HP, Lab Vision Corporation, Fremont, CA, USA) and Ultra V Block (TA-125-UB, Thermo Scientific, Cheshire, UK) solution, they were incubated with Caspase-3 (sc-65497, Santa Cruz, CA, USA, 1:200), VEGF (sc-7269, Santa Cruz, CA, USA, 1:200), TNF alpha (sc-52746, Santa Cruz, CA, USA, 1:200), NFκB p65 (sc-8008, Santa Cruz, CA, USA, 1:200), and IL-1β (sc-52012, Santa Cruz, CA, USA, 1:200) for 60 min. Then they were incubated with secondary antibody (Biotinylated Goat Anti-Polyvalent TP-125-BN, Thermo Scientific, Cheshire, UK) and Strepavidin HRP (Horse radish peroxidase) (TS-125-HR, Thermo Scientific, Cheshire, UK) for 30 min at room temperature. .. After DAB (3,3′-diaminobenzidine) (TA-125 HD, Thermo Scientific, Cheshire, UK) solution, tissues were counter-stained with Mayer’s Hematoxylin and covered with Entellan.



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    Modulation of intracellular signaling pathways in HD, RA, and SLE cells exposed to environmental contaminants. Heatmap showing the relative expression and phosphorylation levels of key signaling proteins involved in immune regulation, inflammation, and cell survival (STAT1, STAT3, p38, AKT, and NFκB) and their phosphorylated forms in HD, RA, and SLE after exposure to PM, silica, and TCDD. Data are represented as z-score normalized values. Asterisks indicate statistically significant differences compared with the unstimulated control within each group (p < 0.05). AKT: protein kinase B; HD: healthy donors; NFκB: nuclear factor kappa-light-chain-enhancer of activated B cells; P: phosphorylated form; p38: p38 mitogen-activated protein kinase; PM: particulate matter; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; STAT: signal transducer and activator of transcription; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin.

    Journal: Journal of Translational Autoimmunity

    Article Title: Exploring the immunomodulatory effects of environmental contaminants on autoimmune patients: An in vitro approach

    doi: 10.1016/j.jtauto.2025.100341

    Figure Lengend Snippet: Modulation of intracellular signaling pathways in HD, RA, and SLE cells exposed to environmental contaminants. Heatmap showing the relative expression and phosphorylation levels of key signaling proteins involved in immune regulation, inflammation, and cell survival (STAT1, STAT3, p38, AKT, and NFκB) and their phosphorylated forms in HD, RA, and SLE after exposure to PM, silica, and TCDD. Data are represented as z-score normalized values. Asterisks indicate statistically significant differences compared with the unstimulated control within each group (p < 0.05). AKT: protein kinase B; HD: healthy donors; NFκB: nuclear factor kappa-light-chain-enhancer of activated B cells; P: phosphorylated form; p38: p38 mitogen-activated protein kinase; PM: particulate matter; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; STAT: signal transducer and activator of transcription; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin.

    Article Snippet: Membranes were stripped and re-probed for total AKT, NFκB p65, p38 MAPK, STAT1, STAT3, and β-actin (Cell Signaling Technology, Danvers, MA, USA; Santa Cruz Biotechnology, Dallas, TX, USA) as a loading control.

    Techniques: Protein-Protein interactions, Expressing, Phospho-proteomics, Control

    Modulation of intracellular signaling pathways in HD, RA, and SLE cells exposed to environmental contaminants. Heatmap showing the relative expression and phosphorylation levels of key signaling proteins involved in immune regulation, inflammation, and cell survival (STAT1, STAT3, p38, AKT, and NFκB) and their phosphorylated forms in HD, RA, and SLE after exposure to PM, silica, and TCDD. Data are represented as z-score normalized values. Asterisks indicate statistically significant differences compared with the unstimulated control within each group (p < 0.05). AKT: protein kinase B; HD: healthy donors; NFκB: nuclear factor kappa-light-chain-enhancer of activated B cells; P: phosphorylated form; p38: p38 mitogen-activated protein kinase; PM: particulate matter; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; STAT: signal transducer and activator of transcription; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin.

    Journal: Journal of Translational Autoimmunity

    Article Title: Exploring the immunomodulatory effects of environmental contaminants on autoimmune patients: An in vitro approach

    doi: 10.1016/j.jtauto.2025.100341

    Figure Lengend Snippet: Modulation of intracellular signaling pathways in HD, RA, and SLE cells exposed to environmental contaminants. Heatmap showing the relative expression and phosphorylation levels of key signaling proteins involved in immune regulation, inflammation, and cell survival (STAT1, STAT3, p38, AKT, and NFκB) and their phosphorylated forms in HD, RA, and SLE after exposure to PM, silica, and TCDD. Data are represented as z-score normalized values. Asterisks indicate statistically significant differences compared with the unstimulated control within each group (p < 0.05). AKT: protein kinase B; HD: healthy donors; NFκB: nuclear factor kappa-light-chain-enhancer of activated B cells; P: phosphorylated form; p38: p38 mitogen-activated protein kinase; PM: particulate matter; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; STAT: signal transducer and activator of transcription; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin.

    Article Snippet: Membranes were blocked with 5 % non-fat milk in TBS-T and incubated overnight at 4 °C with primary antibodies against phospho-AKT (Thr308), phospho-NFκB p65 (Ser536), phospho-p38 MAPK (Thr180/Tyr182), phospho-STAT1 (Tyr701), and phospho-STAT3 (Tyr705) (Cell Signaling Technology, Danvers, MA, USA; Santa Cruz Biotechnology, Dallas, TX, USA).

    Techniques: Protein-Protein interactions, Expressing, Phospho-proteomics, Control

    Modulation of intracellular signaling pathways in HD, RA, and SLE cells exposed to environmental contaminants. Heatmap showing the relative expression and phosphorylation levels of key signaling proteins involved in immune regulation, inflammation, and cell survival (STAT1, STAT3, p38, AKT, and NFκB) and their phosphorylated forms in HD, RA, and SLE after exposure to PM, silica, and TCDD. Data are represented as z-score normalized values. Asterisks indicate statistically significant differences compared with the unstimulated control within each group (p < 0.05). AKT: protein kinase B; HD: healthy donors; NFκB: nuclear factor kappa-light-chain-enhancer of activated B cells; P: phosphorylated form; p38: p38 mitogen-activated protein kinase; PM: particulate matter; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; STAT: signal transducer and activator of transcription; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin.

    Journal: Journal of Translational Autoimmunity

    Article Title: Exploring the immunomodulatory effects of environmental contaminants on autoimmune patients: An in vitro approach

    doi: 10.1016/j.jtauto.2025.100341

    Figure Lengend Snippet: Modulation of intracellular signaling pathways in HD, RA, and SLE cells exposed to environmental contaminants. Heatmap showing the relative expression and phosphorylation levels of key signaling proteins involved in immune regulation, inflammation, and cell survival (STAT1, STAT3, p38, AKT, and NFκB) and their phosphorylated forms in HD, RA, and SLE after exposure to PM, silica, and TCDD. Data are represented as z-score normalized values. Asterisks indicate statistically significant differences compared with the unstimulated control within each group (p < 0.05). AKT: protein kinase B; HD: healthy donors; NFκB: nuclear factor kappa-light-chain-enhancer of activated B cells; P: phosphorylated form; p38: p38 mitogen-activated protein kinase; PM: particulate matter; RA: rheumatoid arthritis; SLE: systemic lupus erythematosus; STAT: signal transducer and activator of transcription; TCDD: 2,3,7,8-tetrachlorodibenzo-p-dioxin.

    Article Snippet: Membranes were stripped and re-probed for total AKT, NFκB p65, p38 MAPK, STAT1, STAT3, and β-actin (Cell Signaling Technology, Danvers, MA, USA; Santa Cruz Biotechnology, Dallas, TX, USA) as a loading control.

    Techniques: Protein-Protein interactions, Expressing, Phospho-proteomics, Control